Intact DNA polymerase α/primase from mouse cells
نویسندگان
چکیده
منابع مشابه
Electron-microscopy of intact nuclear DNA from human cells.
Structures retaining many of the morphological features of nuclei may be released by lysing human cells in a non-ionic detergent and 2 M NaCl. Such nucleoids contain all the nuclear DNA packaged within a flexible cage of RNA and protein. HeLa nucleoids have been spread at an air-water interface and viewed in the electron microscope. A tangled network of superhelical fibres surrounds the collaps...
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A procedure has been devised for the purification of intact DNA polymerase alpha from early embryos of Drosophila melanogaster. The purified enzyme consists of at least three polypeptides with Mrs of 182,000, 60,000, and 50,000. These are related antigenically to the alpha (Mr 148,000), beta (Mr 58,000), and gamma (Mr 46,000) subunits, respectively, of the DNA polymerase described previously [B...
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Just as after 70% hepatectomy, the activity of the 7.1 S DNA polymerase, but not the 3.2 S polymerase, is elevated in liver nuclei from unoperated animals in which hepatic DNA replication has been induced with a mixture of biochemicals or by a dietary manipulation. Again as with regenerating liver, the stimulated intact livers show a relationship between the increases in the enzyme activity and...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)47316-x